Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMKVFLQNRDFRQLTINQWISTLGDTIFYLAFLNYVADASF-APLAILLITISETLPQ-VLQIFLGVLADFQHHRVLKYTVISFAKFLLYSIVSLSLSG-------QSFSLLLVAFICLINLLSDTLSYFSG-----AMLTPIFIRIIGQDHLAEAIGFKQSTVSLVKTISNILGGVLLGILSIQF---ISLLNALTFLIAFLGILFI-KTDLLKVEKTINYQEGLSVKSFCQHLLQSSKLIWNMNKVLLVLFIISTSQAVINVTVPVSTLFLRNQPFLNLQTGQSLALLSTFELSALIVGSLVSGYLQHTISIKTALYASLVIQLL------LLVGF-ATVRFDWILIFSTLDAFFAGVLSPRLQELVFKQIPEESMGAVQSSISAITVVLPSLFTISLVTIATSFGTLAVSFVLLLFLLVAFVMLLNIRESI 5AYN Chain:A ((7-419)) -------LRIETQLLLGRLLTRSGDQAWDFVVPFALLVIFPGKLQVAAFYYLIVKIGTFLLTPSSGKWIDTH-PRIQVVKWGVWLQFFAILAGMVFFGMLDGLVRAGGRESWLLSVLFIALALSGVMASLGSQITDISVGNDLAPSLVAPEKLTHFNSWLRRIDLATEVGAPILAGALF-------PLAGLFLIGLWNLVSFVPEYFLLRNVIQRSGLKIK--VLTEA--------INLRGSFSDPIFWLILSYALLWLSVLSPHGVLLAAYL-KDE-MRLP-ETEIGLFRGLGAVFGLISTVSFPYLVRRLGLISSSRWHLGFQGVTLGIAVTAFAMGSTASVYVFLGCILLSRVGLYGFSNGEFELRQRLIPEGRRGELNSLSSLTTTSATLILFSAGSLLPQTEDFKYLVYVSLAAVLLANVV--------

General information: TITO was launched using: RESULT: Template: 5AYN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1668 -197135 -118.19 -528.51 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -118.19 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.311

(partial model without unconserved sides chains): PDB file : Tito_5AYN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5AYN-query.scw PDB file : Tito_Scwrl_5AYN.pdb: