Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTISLVYISLSG-NTESFVTRLKDYLLSQYKRIEVQKIHIKDLVKEGKNFYEMDHPYVAFLPTYLEGGNGVDNGDVEILTTPVGDFIAYGNNASKCFGVVGSGNRNFNNQYCLTAKQYSQRFGFPVLADFEMRGMLEDIKHVAAIIADLYELEKEN 3N3A Chain:C ((24-152)) -----LVYFSSSSENTQRFIERLG---------LPAVRIPLNE-----RERIQVDEPYILIVPSY--GGGGTAGA----VPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCGVPWLYRFELMGTQSDIENVRKGVTEFWQRQ---

General information: TITO was launched using: RESULT: Template: 3N3A.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 605 -24660 -40.76 -192.65 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain C : 0.76 3D Compatibility (PKB) : -40.76 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.394

(partial model without unconserved sides chains): PDB file : Tito_3N3A.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3N3A-query.scw PDB file : Tito_Scwrl_3N3A.pdb: