Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKMERNINMKKWQTCVLGAGSLLCLTACSGKSVTSEHQTKDEMKTEQTASKTSAAKGKEVADFELMGVDGKTYRLSDYKGKKVYLKFWASWCSICLASLPDTDEIAKEAGDDYVVLTVVSPGHKGEQSEADFKNWYKGLDYKNLPVLVDPSGKLLETYGVRSYPTQAFIDKEGKLVKTHPGFMEKDAILQTLKELS 4HQZ Chain:A ((47-187)) --------------------------------------------------------VGKDAPDFTLQSMDGKEVKLSDFKGKKVYLKFWASWCGPCKKSMPELMELAAKPDRDFEILTVIAPGIQGEKTVEQFPQWFQEQGYKDIPVLYDTKATTFQAYQIRSIPTEYLIDSQGKIGKIQFGAISNADAEAAFKEMN

General information: TITO was launched using: RESULT: Template: 4HQZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 664 -62119 -93.55 -440.56 target 2D structure prediction score : 0.75 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -93.55 2D Compatibility (Sec. Struct. Predict.) : 0.75 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.689

(partial model without unconserved sides chains): PDB file : Tito_4HQZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4HQZ-query.scw PDB file : Tito_Scwrl_4HQZ.pdb: