Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIKKIYPIFTILLGAAIYAFGLTYFVVPHHLFEGGATGITLITFYLFKIPVSLMNLLINIPLFILAWKIFGAKSLYSSLLGTLALSAWLAFFEHIPLHIDLQGDLLITALIAGILLGIGLGIIFNAGGTTGGTDILARILNKYTHISIGKLLFILDFCILMLILLIFKDLRLVSYTLLFDFIVSRVIDLIGEGGYAGKGFMIITKRPDQLAKAINDDLGRGVTFISGQGYYSKENLKIIYCIVGRNEIVKTKEMIHRIDPQAFITITEAHEILGEGFTFEKE 3WC0 Chain:A ((101-127)) -------------------------------------------------------------------------TLFSSLMSTYYMYFWSQYFPDKPLHID--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3WC0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 9 -3545 -393.89 -131.30 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain A : 0.42 3D Compatibility (PKB) : -393.89 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.42 QMean score : 0.543

(partial model without unconserved sides chains): PDB file : Tito_3WC0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3WC0-query.scw PDB file : Tito_Scwrl_3WC0.pdb: