Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQKNIYFVVLDLHT-TDRDKIIQLFKDWTDYSAKLVEGELVKKDGQNALFPPSDTGETVGLNPHRLTLTFGVSASFLKRMNLENKRPRLFRDLPLFPKEQLREKYTGGDIVIHACADDEQIAFHAIRNLIRKGRNAVPLRWSQSGF----AAIGDRMETPWNLFGFKDGTANPTK-EQDFDRVIWADSKD---WMENGSYMAVRRIQMFLETWDRTSLEEQENTFGRYKESGAPF---GKKNEFDEVDLS--------LLPDDSHVCLAK---EVDKPLLRRSYSYSDGIDEKTGQFDTGLLFISFQKDP-DNFVKVQTNLGATDKMNEYITHIGSGLFTCFGGVEKGGYIGQKLLEG 5MJH Chain:A ((26-376)) MQARGHLVAFDLAAGAGRKEAAALLRRWSDTARRLMAGEPAGS---------RDTDVARDAGPSSLTVTFGFGHSFFGRTGLEKQRPVALDPLPDFSSDHLDKNRSNGDLWVQIGADDALVAFHALRAIQRDAGAAARVRWQMNGFNRSPGATAHPM-TARNLMGQVDGTRNPKPGEADFDRRIFVPE--GPAWMANGSYVVVRRIRMLLDDWEELSLKAQEDVIGRRKSDGAPLSGGSGATESTEMDLEKTDGSGELVVPINAHARITRPDQNGGAAMVRRPFSYHDGFDAD-GVPDAGLLFVCWQADPLRGFVPVQRKLDRGDALSQFIRHEASGLFAVPGGAAEGEYVGQRLLE-

General information: TITO was launched using: RESULT: Template: 5MJH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1609 25228 15.68 78.84 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : 15.68 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.440

(partial model without unconserved sides chains): PDB file : Tito_5MJH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5MJH-query.scw PDB file : Tito_Scwrl_5MJH.pdb: