Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTVEEEKAFLARHLKATEAGEFVTIDALFQAYKKELGRSYTRDAFYQLLKR----------HGWRNITPRPEHPRKADAQTIVASKNKISIQEDKKAL 1E19 Chain:A ((123-179)) -------------------------DPAFQNPTKPVGPFYDEETAKRLAREKGWIVKEDSGRGWRRVVPSPDPKGHVEAETI----------------

General information: TITO was launched using: RESULT: Template: 1E19.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 145 10158 70.06 216.13 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : 70.06 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.393

(partial model without unconserved sides chains): PDB file : Tito_1E19.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1E19-query.scw PDB file : Tito_Scwrl_1E19.pdb: