Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEQFLDNIKDLEVT--TVVRAQEALDKKETATFFIGRKTCPYCRKFAGTLSGV---VAETKAHIYFI---NSEE----------------PSQLNDLQAFRSRYGIPTVPGFVHI-TDGQI---NVRCDSSMSAQEIKDFAGL 4FYU Chain:A ((2-133)) -ADLLANI-DLKKADGTVKKGSDALANKKVVALYFSAHWCPPCRQFTPILKEFYEEVDDDQFEIVFVSLDHSEEDLNNYVKESHGDWYHVPFGSSEIEKLKNKYEVAGIPMLIVIKSDGNVITKNGRADVSGKA---------

General information: TITO was launched using: RESULT: Template: 4FYU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 404 5080 12.57 48.85 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : 12.57 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.449

(partial model without unconserved sides chains): PDB file : Tito_4FYU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4FYU-query.scw PDB file : Tito_Scwrl_4FYU.pdb: