Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEKKIVKDILFLSQVSQPASQEDLYLARDLQDTL--LANRDTCVGLAANMIGVQKRVIIFNLGLVPVVMFNPVLLSFEGSYE-AEEGCLS----LVGVRSTKRYETIRLAYRDSKWQEQTITLTGFPAQICQHELDHLEGRII 1LME Chain:A ((12-139)) -----------LRKRAKPVTKFDENLKKTIERMIETMYHYD-GVGLAAPQVGISQRFFVMDVGNGPVAVINPEILEIDPETEVAEEG-LSFPEIFVEIERSKR---IKVKYQNTRGEYVEEELEGYAARVFQHEFDHLNGVLI

General information: TITO was launched using: RESULT: Template: 1LME.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 535 -24341 -45.50 -202.84 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -45.50 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.463

(partial model without unconserved sides chains): PDB file : Tito_1LME.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1LME-query.scw PDB file : Tito_Scwrl_1LME.pdb: