Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQSYSVIVVRSQEKKDALYELVPQEAIRQSAV-FLLFVGDLNRAEKGARLHTDTFQPQGVEGLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYPVFGMALGVPNQTHDVKPRLPLDNVVFEEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQF-GQAEPSSTRKNLEQKKLL 5UU6 Chain:A ((7-229)) ---TIQTILGHRSIRKFTSQPIDKEQLETILQAGLAASSSSMLQVVSIVRVTDIEKRSQLAKFAGNQAYVESAAEFLVFCIDYQRH---ASINPDV-QADFTELMLIGAVDSGIMAQNCLLAAESMGLGGVYIGGLRNNAQQVDELLGLPQNTAILFGMCLGHPDQNPEVKPRLPAHVVVHENQYQDLNLDDIQAYDQTMQNYYA----STWSQEVTSKLAGESRP-------------

General information: TITO was launched using: RESULT: Template: 5UU6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 859 -67263 -78.30 -315.79 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.81 3D Compatibility (PKB) : -78.30 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.554

(partial model without unconserved sides chains): PDB file : Tito_5UU6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5UU6-query.scw PDB file : Tito_Scwrl_5UU6.pdb: