Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSFQEELNQLLRQYVGRETPLYYAKNLTQHIGGAKIYLKREDLNHTGAHKINNALGQVWLAKRMGKKKIIAETGAGQHGVATATAAALFNMECIIYMGEEDVKRQALNVFRMELLGAKVEAVTDGSRVLKDAVNAALRSWVANIDDTHYILGSTLGPHPFPEIVRDFQSVIGREAKQQYRDLTGQNLPDALVACVGGGSNAIGLFHPFVEDESVAMYGAEAAGLGVDTEHHAATLTKGRPGVLHGSLMDVLQDAHGQILEAFSISAGLDYPGIGPEHSHYHDIKRASYVPVTDEEALEGFQLLSRVEGIIPALESSHAIAFAVKLAKELGPEKSMIVCLSGRGDKDVVQVKDRLEADAAKKGEAHA 5EY5 Chain:B ((1-381)) ---------GRFGKYGGQYVPETLMPALEELEEAYERAKNDPEFQAELEYYLRDYVGRPTPLYFAENLTKDLGGAKIYLKREDLNHTGAHKINNALGQALLAKRMGKKRVIAETGAGQHGVATATVAAMFGLECVVYMGAEDIERQALNVFRMKLLGAKVRPVTSGSRTLKDAINEAMRDWVTNVEDTFYIIGSVVGPHPYPMMVRDFQSVIGEEARQQILEKEGR-LPDAIVACVGGGSNAMGIFHPFIDDESVRLIGVEAAGKGIETGKHAATLSAGRPGVLHGAMTYLLQDEDGQIIEAHSISAGLDYPGVGPEHAYLKDTGRAEYVSVTDDEALEAFQLLSRTEGIIPALESSHAVAYAMKLAPELSKDQIIVVNLSGRGDKDVNTV----------------

General information: TITO was launched using: RESULT: Template: 5EY5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2630 -160501 -61.03 -421.26 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain B : 0.92 3D Compatibility (PKB) : -61.03 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.92 QMean score : 0.502

(partial model without unconserved sides chains): PDB file : Tito_5EY5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5EY5-query.scw PDB file : Tito_Scwrl_5EY5.pdb: