Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQETKLSA-KGPTKKHVEILEELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTISFPEIGAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDAKYAEYLAELDKEKPVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLL-ATGFTDTFRHVHGDVPERYTWWAQRSKTSKINNTGWRIDYWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL 1DEW Chain:A ((23-279)) LKICSWNVDGL---------RAWIKKKGLDWVKEEAPDILCLQETKCSENKLPAE-----LQEL-PGLSHQYWSAPS-DKEGYSGVGLL-SRQCPLKVSY-GIGD-EEHDQEGRVIVAEFDSFVLVTAYVPNAGRGLVRLEYRQRWDEAFRKFLKGLASRKPLVLCGDLNVAHEEIDLRNPKGNKKNAGFTPQERQGFGELLQAVPLADSFRHLYPNTPYAYTFWTYM-MNARSKNVGWRLDYFLLSHSLLPALCDSKIRSKALGSDHCPITLYLAL

General information: TITO was launched using: RESULT: Template: 1DEW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1455 -19708 -13.55 -77.29 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : -13.55 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.464

(partial model without unconserved sides chains): PDB file : Tito_1DEW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1DEW-query.scw PDB file : Tito_Scwrl_1DEW.pdb: