Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKYQEETMKDFHFDAISAFENYEIEKMRDGHVVVTTKVVNSSLNYYGNAHGGYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGKLDDVLTIKGECVHQGRTTCVMDVDITNQEGRNVCKATFTMFVTGQRSEERRVSI 4ZRF Chain:A ((5-123)) -----------HFDAISAFENYEIEKMRDGHVVVTTKVVNSSLNYYGNAHGGYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGKLDDVLTIKGECVHQGRTTCVMDVDITNQEGRNVCKATFTMFVT-----------

General information: TITO was launched using: RESULT: Template: 4ZRF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 601 -75682 -125.93 -635.98 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.94 3D Compatibility (PKB) : -125.93 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.94 QMean score : 0.449

(partial model without unconserved sides chains): PDB file : Tito_4ZRF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZRF-query.scw PDB file : Tito_Scwrl_4ZRF.pdb: