Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSTLAKIEALLFVAGEDGIRVRQLAELLS--LPPTGIQQSLGKLAQKYEKDPDSSLALIETSGAYRLVTKPQFAEILKEYSKAPINQSLSRAALETLSIIAYKQPITRIEIDAIRGVNSSGALAKLQAFDLIKEDGKKEVLGRPNLYVTTDYFLDYMGINHLEELPVIDELEIQAQESQLFGERIEEDENQ 1T6S Chain:A ((9-158)) ---LRSLEALIF-SSEEPVNLQTLSQITAHKFTPSELQEAVDELNRDYEAT-GRTFRIHAIAGGYRFLTEPEFADLVRQLLAPVIQRRLSRSMLEVLAVVAWHQPVTKGEIQQIRGASPDYSIDRLLARGLIEVRGRADSPGRPLQYGTTEVFLD------------------------------------

General information: TITO was launched using: RESULT: Template: 1T6S.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 452 7213 15.96 48.73 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : 15.96 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.602

(partial model without unconserved sides chains): PDB file : Tito_1T6S.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1T6S-query.scw PDB file : Tito_Scwrl_1T6S.pdb: