Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSKLLDKILSRENMLEAYNQVKSNKGSAGIDGMTIEEMDNYLRQNWRLTKELIKQRKYKPQPVLRVEIPKPDGGIRQLGIPTVMDRMIQQAIV 5IRG Chain:A ((17-107)) --SLMEQILSNDNLNRAYLQVVRNKGAEGVDGMKYTELKEYLAKNGEIIKEQLRIRKYKPQPVRRVEIPKPDGGV-NLGVPTVTDRFIQQAIA

General information: TITO was launched using: RESULT: Template: 5IRG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 270 -27483 -101.79 -305.37 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.87 3D Compatibility (PKB) : -101.79 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.87 QMean score : 0.683

(partial model without unconserved sides chains): PDB file : Tito_5IRG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5IRG-query.scw PDB file : Tito_Scwrl_5IRG.pdb: