Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKKILASLLLSTVMVSQVAVLTTAHAETTDDKIAAQDNKISNLTAQQQEAQKQVDQIQEQVSAIQAEQSNLQAENDRLQAESKKLEGEITELSKNIVSRNQSLEKQARSAQTNGAVTSYINTIVNSKSITEAISRVAAMSEIVSANNKMLEQQKADKKAISEKQVANNDAINTVIANQQKLADDAQALTTKQAELKAAELSLAAEKATAEGEKASLLEQKAAAEAEARAAAVAEAAYKEKRASQQQSVLASANTNLTAQVQAVSESAAAPVRAKVRPTYSTNASSYPIGECTWGVKTLAP-----WAGDYWGNGAQWATSAAAAGFRTGSTPQVGAIACWN------DGGYGHVAVVTAVESTTRIQVSESNYAG-----NRTIGNHRGWFNPTTTSEGFVTYIYAD 5T1Q Chain:A ((243-345)) -----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------SDSMPYPHGQCTWYVYNRMKQFGTSISGD-LGDAHNWNNRAQYRDYQVSHTPKRHAAVVFEAGQFGADQHYGHVAFVEKVNSDGSIVISESNVKGLGIISHRTI-----------------------

General information: TITO was launched using: RESULT: Template: 5T1Q.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 328 -510 -1.55 -5.86 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -1.55 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.473

(partial model without unconserved sides chains): PDB file : Tito_5T1Q.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5T1Q-query.scw PDB file : Tito_Scwrl_5T1Q.pdb: