Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLFSGSSMSEQIFIQGPVGKIELFVDRPEGEIKGFAVVCHPHPLQGGTPQHKVPALLTQIFNEYGCIVYRPSFRGLGGSEGVHDEGHGETEDILAVIEHVRKLHA-GLPFYAGGFSFGSHVLAKCHAQLSPELQPVQLILCGLPTATVVGLRHYKTPEIQGDILLIHGEQDDITLLSDA---IEWAKPQKHPI---TILSGANHFFTGYLKQLRQIITRFIIMK 2I3D Chain:A ((25-221)) ----------EVIFNGPAGRLEGRYQPSKEKSAPIAIILHPHPQFGGTMNNQIVYQLFYLFQKRGFTTLRFNFRSIGRSQGEFDHGAGELSDAASALDWVQSLHPDSKSCWVAGYSFGAWIGMQLLMR-RPEIEGFMSI---APQPNTYDFSFLAPCPSSG--LIINGDADKVAPEKDVNGLVEKLKTQKGILITHRTLPGANHFFNGKVDEL-----------

General information: TITO was launched using: RESULT: Template: 2I3D.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1110 -68934 -62.10 -362.81 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -62.10 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.439

(partial model without unconserved sides chains): PDB file : Tito_2I3D.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2I3D-query.scw PDB file : Tito_Scwrl_2I3D.pdb: