Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTETTIQGLAASLNPARAARKIIGFVPTMGNLHEGHLTLVREAKKLCDVVVVSIFVNPTQFGPGEDFDNYPRTLEQDSRLLADVGCDIIFAPSVEQMYGTQPRLTNISVSQITDDLCGSSRPGHFDGVALVVTKLFNIVQPNYAFFGQKDYQQLAVIRQFVQDLNIPLEVIGVPIVRAEDGLALSSRNGYLTPEQRQVAPVIYQGLKQAEEQLHQG----KDLQQVLADLKTLLTDNGFVVDYVEARQPNLLA-ASQFDRDIVLFVAAKLGGTRLIDNLQVAFTPQ 5UCR Chain:A ((27-284)) -----------------------VAFVPTMGNLHEGHLALVREARKRADNVVVSIFVNRLQFGQ--------RTLQQDADKLAAEGVAVVFAPDEKELYPNVEQRYNVEPPHLQNELCGKFRPGHFRGVATVVSKLFNIVLPDVACFGKKDYQQLAVIKGLTEDLNFDIEIVPVDTGRAADGLALSSRNRYLSVGERAEAPRLYRELQAVAESLKQGGLDYAGLERQAAD---HLTAAGWLVDYVEIRRADTLEMARAGDKKLVVLAAARLGTTRLIDNVEVGL---

General information: TITO was launched using: RESULT: Template: 5UCR.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1226 -121297 -98.94 -495.09 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.81 3D Compatibility (PKB) : -98.94 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.616

(partial model without unconserved sides chains): PDB file : Tito_5UCR.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5UCR-query.scw PDB file : Tito_Scwrl_5UCR.pdb: