Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVMFTLKQFQYFIKIVEEGSFTAASEKLFIAQSALSRQMKLLEEEIDFLLFDRTDKRVKLTAAGEVFYKKIKDNLLYLNEIIDLSKSVSEGKNRQIKIAHSSSIVVDNTKVQILKEVSLTQQLSFEINTLSSEQQILALLNGEIDIGLIR-----PPVRHTLDGINTLKLYEEPLMVAVHIDHA-KFAKSEKVYLKDLKDECFVSTPHAERGGLSYLVANLCLAAGFTPQKASIQSRKIS-QLQLVAANLGVGIVPAEFQQILPANVKLIPLEDSLSLSEVLLVYRK-DHDEIIQHCAEQIHQMFLS 2H9B Chain:A ((89-293)) -------------------------------------------------------------------------------------------E-KTIRIGFVGSLLFGLLPRIIHLYRQAHPNLRIELYEMGTKAQTEALKEGRIDAGFGRLKISDPAIKHSL-------LRNERLMVAVHASHPLNQMKDKGVHLNDLIDEKILLYPSSPKPNFSTHVMNIFSDHGLEPTKIN-EVREVQLALGLVAAGEGISLVPASTQSIQLFNLSYVPLLDPDAITPIYIAVRNMEESTYIYSLYETIRQIY--

General information: TITO was launched using: RESULT: Template: 2H9B.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 997 -13116 -13.16 -66.58 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -13.16 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.447

(partial model without unconserved sides chains): PDB file : Tito_2H9B.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2H9B-query.scw PDB file : Tito_Scwrl_2H9B.pdb: