Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEINPNSQQPTGSSLPTSALGNIQRPGEYLRQIRVSQKKELEQVSSDLNMPLKTLNALEQDDYKSLPEATFIKGYYRSYAKYLNTDATAIIQRFDEIYANDTGLLPNHALNNSPIKIMGKLPGSNSDRNKKWLKRALLAILIIAVVSLIVMGVQKWTSNKEDAELPKANQSNVEVLPMKGNASATVGDQLVLNFNRPTSVHIVDATGKVLATGRQASTLTLNGESPFQIRLDDATAVSLSLNQEQISLSPYTVNGKAEFRLSR 1EGZ Chain:A ((53-187)) ------------SSIVRAAMG-VQESGGYL-QDPAGNKAKVERVVDA---------AIANDMYA-------IIGWHSHSAENNRSEAIRFFQEMARKYGNKPNVI--YEIYNEPLQV------SWSNTIKPYAEAVISAIRAIDPDNLIIVGTPSWSQNVDEASRDPINAKNI------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1EGZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 617 -41269 -66.89 -305.69 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -66.89 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.260

(partial model without unconserved sides chains): PDB file : Tito_1EGZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1EGZ-query.scw PDB file : Tito_Scwrl_1EGZ.pdb: