Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVVAQQLGQWAEQTALKLLKEQNYEWVASNYHSRRGEVDLIVKRGNELIFVEVKA---RGQGNYGQACEMVTLSKQKKIIKT-------------AMRFLQRYPSYQDFYCRFDVICFDFPQKIAKTVQQDFSKFHYDLQWIENAFTLD 1GUD Chain:A ((126-213)) -------------------------------------IDKLGAEGGEVAIIEGKAGNASGEARRNGATEAFKKASQIKLVASQPADWDRIKALDVATNVLQRNPNIKAIYCANDTMAMGVAQAVA------------------------

General information: TITO was launched using: RESULT: Template: 1GUD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 221 -11484 -51.96 -159.49 target 2D structure prediction score : 0.33 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -51.96 2D Compatibility (Sec. Struct. Predict.) : 0.33 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.381

(partial model without unconserved sides chains): PDB file : Tito_1GUD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1GUD-query.scw PDB file : Tito_Scwrl_1GUD.pdb: