Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKYCQFFSVLALGLSAASCAVTSGLQTYDIPSEGVYKTDLGTTVNVVKISQETLPAIQP----AQIDYQRDYASLFKNQQTIYRLSPGDVLSIQLWAYPEIAPPVNNISNEQSVQAYGYPIDQTGYIQFPLVGRYKAAGKTLAQVNRELHSQLARFLKNPDVVVRVVSYEGQRFSVQGSVTKGGQFYLSDQPVSIYTALGMAGGVTTTGDNTYIQLIRNGRTYNLNTIDLEKAGYSLHKLLVQPNDTIYVSTRENQKIYVMGESGKNQALPMRDQGMSLTDALGESLGINPLSGSASRIYVVRTNPNDRT---TEIYHLNLMSIGDFGLANQFRLRSNDVVYVDATGLTRWQRIVNQIIPFSNALYNIDRLGQ 2W8H Chain:A ((29-326)) --------------------------------------DLDKLVNVYPMTPGLIDQLRPEPVIARSNPQLD--NLLKSYE--YRIGVGDVLMVTVWDHPELTTPAGQY---RSASDTGNWVNSDGTIFYPYIGKVQVAGKTVSQVRQDITSRLTTYIESPQVDVSIAAFRSQKVYVTGEVANSGKQAITNIPLTVMDAINAAGGLAADADWRNVVLTHNGKDTKISLYALMQKGDLTQNHLLYHGDILFIPSNDDLKVFVMGEVGKQSTLKMDRSGMTLAEALGNAEGISQEMSDATGIFVVRQLKGDRTGKIADIYQLNAQDASAMVLGTEFQLCPYDIVYV------------------------------

General information: TITO was launched using: RESULT: Template: 2W8H.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1267 1108 0.87 3.81 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : 0.87 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.519

(partial model without unconserved sides chains): PDB file : Tito_2W8H.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2W8H-query.scw PDB file : Tito_Scwrl_2W8H.pdb: