Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MANSAQAKKRARQNVKARKHNASLRSMVRTYIKRTLSAIAGGDYAVATEAYKKAVPVIDRMADKGIIHKNKAARHKSRLNAQVKALAN 5UZ4 Chain:T ((3-87)) --NIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDKAAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA-

General information: TITO was launched using: RESULT: Template: 5UZ4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain T - contact count / total energy / energy per contact / energy per residue : 187 -17602 -94.13 -207.08 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain T : 0.85 3D Compatibility (PKB) : -94.13 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.728

(partial model without unconserved sides chains): PDB file : Tito_5UZ4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5UZ4-query.scw PDB file : Tito_Scwrl_5UZ4.pdb: