Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTDITLKTPVEMRNVQLKVYDNTDNSILIDT-QVASLTS-YDIKLPSTKLNRLYRIEVTT-QGSSQVFDPTKSEYQNISGVYHAFITPSSISNKTQLISPSSEAIYQRAVIRSGQLPNETIIPTRIEQLHVDLASQDVYRSLLNAFKDTNIPSLSPASTLTILNLLKYSTTKPSTYVDSYLCFGYLQYWSSIHNTNSPYQDLTQSLATDLKDGYLDGKKLIGDKTSFTPIFTSAPDNIDPAKNTLLDIAANQKVTRDYFATNLRAATFKLADLQDQSHLDPIGYDLLDKKQYSGIIPAGNTSEFFRIDGAGDYRRAVGFSGITATCNGSAYPCKQGLTSINISDPKLPATDYLVGHYEDSTTGCQLNFRANGQIELIKGSQSYRSALSADSTDKLLQTDAASYSYLLNSSSSEPNNSTQQYNFIQLHIKANKIVSAQAGLDSRKAPDVLQTTQLEYSFS 3D33 Chain:A ((66-130)) ---LTLKNASPDRDMTIRITDMAKGGVVYENDIPEVQSAYITISIANF-PAEEYKLEITGTPSGHLTGY------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3D33.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 209 -11619 -55.59 -187.40 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.54 3D Compatibility (PKB) : -55.59 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.54 QMean score : 0.502

(partial model without unconserved sides chains): PDB file : Tito_3D33.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3D33-query.scw PDB file : Tito_Scwrl_3D33.pdb: