Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTDQYQAFTQSPIGKFVVKNLGLPSPVVLERFESAQPVVKGAVLVGAAPSSVLSGAIAQVLSNIHADSYVGNNVALQQEAAKVGLNLRPFNAGDKESKFKAVVFDASGIQNSEQLNELYKFFNPIARQVATSGRVIVIGTTPETAKTIKQAIAQRALEGFIKSVGKEFKKGITAQVVYVD---EGAAANLESTLRFLLSPRSAYVSGQVIRVSKADVVD-VDWAKPLAGKTALVTGASRGIGEAIAHVLARDGAHVICLDVPQQQADLDRVAADIGGSTLAIDITAADAGEKIKAAAAKQGG--LDIIVHNAGITRDKTLANMKPELWDLVININLSAAERINDYLLENDGLNANGRIVCVSSISGIAGNLGQTNYAASKAGVIGLVKFTAPILK-NGITINAVAPGFIETQMTAAIPFAIREAGRRMNSMQQGGLPVDVAETIAWFASTASTGVNGNVVRVCGQSLLGA 4FW8 Chain:A ((20-454)) --------------SFLARQLGVPQPETLRRYRAGEPPLTGSLLIGGA---------GRVVEPLRA--------ALEKDYDLVG------------DSFGGLVFDATGITEPAGLKGLHEFFTPVLRNLGRCGRVVVVGGTPEAAASTNERIAQRALEGFTRSLGKELRRGATTALVYLSPDAKPAATGLESTMRFLLSAKSAYVDGQVFSVGADDSTPPADWEKPLDGKVAIVTGAARGIGATIAEVFARDGAHVVAIDVESAAENLAETASKVGGTALWLDVTADDAVDKISEHLRDHHGGKADILVNNAGITRDKLLANMDDARWDAVLAVNLLAPLRLTEGLVGNGSIGEGGRVIGLSSIAGIAGNRGQTNYATTKAGMIGITQALAPGLAAKGITINAVAPGFIETQMTAAIPLATREVGRRLNSLLQGGQPVDVAEAIAYFASPASNAVTGNVIRVCGQAMIGA

General information: TITO was launched using: RESULT: Template: 4FW8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2464 -110389 -44.80 -262.83 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.85 3D Compatibility (PKB) : -44.80 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.551

(partial model without unconserved sides chains): PDB file : Tito_4FW8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4FW8-query.scw PDB file : Tito_Scwrl_4FW8.pdb: