Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNPSISLIREIDALLPQTQCGLCGHRDGCLPYAKSIAEGEEANKCVPGGQPVADALAQLLNRPLLPAETSVWPVQADGRPQRMKAIIREDECIGCTKCINAC---PVDAIIGSGKLMHTILTDLCTGCELCIPPCP-VDCIDLVEDTQALPSEQQRFAEQNDLRQRYYAHIQREEKRRIHRKGPVVRAEIDTQLFAQFSQALDDLPSIQLIEKPKEVIVSDAKTTIELAKIRTQIKKLEKQLSVREDAKKRLQLDELQQQLIQLQEV 1GT8 Chain:A ((942-1015)) --------------------------------------------------------------------------------EQVVAVIDEEMCINCGKCYMTCNDSGYQAIQFDPETHLPTVTDTCTGCTLCLSVCPIIDCIRMVSRTT--PYEPKR---------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1GT8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 287 -26208 -91.32 -374.39 target 2D structure prediction score : 0.43 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -91.32 2D Compatibility (Sec. Struct. Predict.) : 0.43 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.451

(partial model without unconserved sides chains): PDB file : Tito_1GT8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1GT8-query.scw PDB file : Tito_Scwrl_1GT8.pdb: