Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAIQRSGRFERIKKPLKSDMNVVPYIDVMLVLLVIFMVTAPMITSGIKVDLPQANNHPIESKDMPAMVTIGKDGNIYLQYEDYKDNEPLSLEKLESVLSEAQKKAEQENKQLTVLVNGDKTRPYGEVVSLMASLQEAGLTQVGLLTEPLK 2PFU Chain:A ((45-135)) ----------------------------------------------VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIG------NDPVTDETMITALNALT----EGKKDTTIFFRADKTVDYETLMKVMDTLHQAGYLKIGLVGE---

General information: TITO was launched using: RESULT: Template: 2PFU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 242 -30516 -126.10 -335.34 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -126.10 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.571

(partial model without unconserved sides chains): PDB file : Tito_2PFU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2PFU-query.scw PDB file : Tito_Scwrl_2PFU.pdb: