Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLSQFVNKTLKVLALCTGLISTSHFAQANDFVTHPNYLNFKQKAMSTYGLSGEQVDAAMNGAKNLPNIINIM---TRPGESKPWYDYRSMFLVEGTIQRGVRFKNQYADALNRAEQQFGVSQAVILGILGVETGYGANKGSFITRDALATLAFGYPRRAEYFGDELAALIAWTYKEGYPTNSIVGSYAGAIGFPQFMPSNISKYGVDFDGNGHIDLRNSAEDAIGSIANYLAKQGWQCDQPIGFIARYTGSNPESIIAKDLTQPTPYGALKNQGISPLNPLVKIDDLDLVNVIQLQDYNGPIYYLTYPNFQVITTYNKSRMYATAVWLLGTEVASR 1QUT Chain:A ((14-317)) -----------------------------DFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQ---GPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIGVETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFAGAMGYGQFMPSSYKQYAVDFSGDGHINLWDPV-DAIGSVANYFKAHGWVKGDQVAVMA--NGQAPG--LPNGFKTKYSISQLAAAGLTPQQPL---GNHQQASLLRLDVGTGYQYWYGLPNFYTITRYNHSTHYAMAVWQLGQAVA--

General information: TITO was launched using: RESULT: Template: 1QUT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1506 -49893 -33.13 -171.45 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -33.13 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.576

(partial model without unconserved sides chains): PDB file : Tito_1QUT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1QUT-query.scw PDB file : Tito_Scwrl_1QUT.pdb: