Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKQLTVDEAAKEILAYQQITF-LTGAGVSTPSGVPDYRSLSGIYHGMEQP----EYLLSHQAMVNEPDKFYQFVKKIYHP--EAKPNIIHLEIAHLAKEKNVW--VVSQNIDGLHAKAKSPQLVNFHGSLYQCHCRKCQQ--TVD--WKEYLHSDKH--QACGGQIRPDIVLYGEGFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCD--LVQFRQPSAKILVIN--QTPVYLAEPYTFIQEDGAVVFKKIQELGADYD 2H4J Chain:A ((8-219)) -----------DLLNESRLTVTLTGAGISTPSGIPDF----------SQNVFDIDFFYSH------PEEFYRFAKEGIFPMLQAKPNLAHVLLAKL-EEKGLIEAVITQNIDRLHQRAGSKKVIELHGNVEEYYCVRCEKKYTVEDVIKKLESSDVPLCDDCNSLIRPNIVFFGENLPQDALREAIGLSSRASLMIVLGSSLVVYPAAELPLITVRS-GGKLVIVNLGETP-----------------------------

General information: TITO was launched using: RESULT: Template: 2H4J.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 883 -8037 -9.10 -43.92 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -9.10 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.511

(partial model without unconserved sides chains): PDB file : Tito_2H4J.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2H4J-query.scw PDB file : Tito_Scwrl_2H4J.pdb: