Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MREKHYFNQDRNFWINV---QQNDVLAVKNLKEEYGISDEMLTYSLDKNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTT-------PNTEYVIRLIRSLL-ERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLLQLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYR---KLSEKEK----EELDDALIEARQAVE-MTNLASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVFGWGIALIISLVLSIWMLIALWRRIR 5JTG Chain:A ((83-370)) -------------WINITGIHRTDV--VQRVGEFFGIHPLVLEKILNVHQRPKVEF--FENYVFIVLKMFTYDKNLHELESEQVSLILTKNCVLMFQEKIGDVFDPVRER-IRYNRGIIRKKRADYLLYSLID----ALVDDYFVLLEKIDDEIDVLEEEVLERPEKETVQRTHQLKRNLVEL----RKTIWPLREVLS-SLYRDVPPLIEKETVPYFRKVYDHTIQIADTVETFRDIVSGLLD----VYLSSVSNKTNEVMKVLTIIATIFMPLTFIAGIY-------------GYPVVLAVMGVIAVIMVVYFKKK--

General information: TITO was launched using: RESULT: Template: 5JTG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 977 16806 17.20 65.90 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : 17.20 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.457

(partial model without unconserved sides chains): PDB file : Tito_5JTG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5JTG-query.scw PDB file : Tito_Scwrl_5JTG.pdb: