Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYKKKVGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN 2KJK Chain:A ((48-75)) IHSKKVGDTVKIKYKHGNKNEEASIKLT--------------

General information: TITO was launched using: RESULT: Template: 2KJK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 44 262 5.94 9.34 target 2D structure prediction score : 0.75 Monomeric hydrophicity matching model chain A : 0.82 3D Compatibility (PKB) : 5.94 2D Compatibility (Sec. Struct. Predict.) : 0.75 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.831

(partial model without unconserved sides chains): PDB file : Tito_2KJK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2KJK-query.scw PDB file : Tito_Scwrl_2KJK.pdb: