Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEWMRFFDNRINYDILITKRKRILSVGGRKVKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDALQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFIN----------GKDR--YTPEELWYKGKKSIPEL 4LHD Chain:A ((423-493)) ------------------------------------------------------------------------------KAAEGRGINLRPL-----VPGEVGISLDETVTVQDLLDLWQVFAGKDNLPFTPEELWSEVKTSFP--

General information: TITO was launched using: RESULT: Template: 4LHD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 87 4093 47.04 85.26 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 47.04 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.280

(partial model without unconserved sides chains): PDB file : Tito_4LHD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4LHD-query.scw PDB file : Tito_Scwrl_4LHD.pdb: