Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYTGISANERKEGARNMEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNEKRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHGGAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTVQGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPT--GEVVSVSGTAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVKTDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVREFVTEYKINIKK 1MMU Chain:A ((2-339)) -----------------SIKIRDFGLGSDLISLTNKAGVTISFTNLGARIVDWQ----KDGKHLILGFDSAKEYLEKDAYPGATVGPTAGRIKDGLVKISGKDY-ILNQNEGPQTLHGGEESIHTKLWTYEVTDLGAEVQVKFSLVSNDGTNGYPGKIEMSVTHSFDDDNKWKIHYEAISDKDTVFNPTGHVYFNLNGDASESVENHGLRLAASRFVPL-KDQTEIVRGDIVDIKNTDLDFRQEKQLSNAFNSNMEQVQLVKGIDHPFLLDQLGLDKEQARLTLDD--TSISVFTDQPSIVIFTANFGDLGTLYHEKKQVHHGGITFECQVSPGSEQIPELGDISLKAGEKYQATTIYSLHTK-

General information: TITO was launched using: RESULT: Template: 1MMU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2153 -50358 -23.39 -149.88 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.81 3D Compatibility (PKB) : -23.39 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.470

(partial model without unconserved sides chains): PDB file : Tito_1MMU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1MMU-query.scw PDB file : Tito_Scwrl_1MMU.pdb: