Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNYYGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVIPQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDVQTLTDNSIKEVDKIAADKEKELLEV 1WQG Chain:A ((2-185)) -IDEALFDAEEKMEKAVAVARDDLSTIRTGRANPGMFSRITIDYYGAATPITQLASINVPEARLVVIKPYEANQLRAIETAIRNSDLGVNPTNDGALIRVAVPQLTEERRRELVKQAKHKGEEAKVSVRNIRRKAMEELHRIRKEGEAGEDEVGRAEKDLDKTTHQYVTQIDELVKHKEGELLEV

General information: TITO was launched using: RESULT: Template: 1WQG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 718 -78876 -109.86 -428.67 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain A : 0.89 3D Compatibility (PKB) : -109.86 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.89 QMean score : 0.638

(partial model without unconserved sides chains): PDB file : Tito_1WQG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1WQG-query.scw PDB file : Tito_Scwrl_1WQG.pdb: