Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAKQARKRILIGGGSEQPMRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSSTKTAS---TTEQTMATSNNEK----KTALDQLKEQQPNVPM-PL-DVPVSSGYLNIAATHTKQGY-SILYYRTDRP------LGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSRITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFGKITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL 5FR0 Chain:A ((75-202)) -----------------------------------------------------TNSSPVNNVTLSSSDFINENGDLISSNNIKISWLKETLANIGRSNPSAPLEPFPDIIHNSGSLNIEKNKIASAWINIKIPRNAKPGIYNGSIEVTADELEK-----SYTFDYSF----EVLNLVQPLPSETNTQ--------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5FR0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 385 41694 108.30 382.51 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : 108.30 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.116

(partial model without unconserved sides chains): PDB file : Tito_5FR0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5FR0-query.scw PDB file : Tito_Scwrl_5FR0.pdb: