Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYDVIVIGAGPSGMTAALYASRSNLSVLMIEQGAPGGQMNNTAEIENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKTVIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLTQFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVKTGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVREKDLRQITTAVGDGGIAGQQVYKYLESLEETTA 5MIP Chain:A ((25-326)) -YDVVIIGSGPAGMTAAMYTARSEMKTLLLERGVPGGQMNNTAEIENYPGYETIMGPELSMKMAEPLEGLGVENAYGFVTGIEDHGDYKKIITEDDEFITKSIIIATGANHRKLEIPGEEEYGARGVSYCAVCDGAFFRNQEILVIGGGDSAVEEALYLTRFGQSVTIMHRRDKLRAQEIIQQRAFKEEKINFIWDSVPMEIKGDDKKIQSVVYKNVKTGEVTEKAFGGIFIYVGLDPVAEFVSDLGITDEAGWIITDDHMRTNIPGIFAVGDVRQKDFRQITTAVGDGAQAAQEAYKFVVEL-----

General information: TITO was launched using: RESULT: Template: 5MIP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1807 -200825 -111.14 -664.98 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.90 3D Compatibility (PKB) : -111.14 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.90 QMean score : 0.560

(partial model without unconserved sides chains): PDB file : Tito_5MIP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5MIP-query.scw PDB file : Tito_Scwrl_5MIP.pdb: