Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMTRR------DFEKKLKKVIEKNAEKE 5LN3 Chain:J ((356-397)) --------------------------GAEVKGVCTEAGMYALRERRVHVTQEDFEMAVAKVMQKDSEK-

General information: TITO was launched using: RESULT: Template: 5LN3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain J - contact count / total energy / energy per contact / energy per residue : 74 5748 77.67 159.65 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain J : 0.69 3D Compatibility (PKB) : 77.67 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.184

(partial model without unconserved sides chains): PDB file : Tito_5LN3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5LN3-query.scw PDB file : Tito_Scwrl_5LN3.pdb: