Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYGNELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNRMTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLL-------------LLILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWLMYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK 3GWU Chain:A ((243-336)) -------------------------------------------------------------------------------------------------------------------------VWIAAVGQIFFTLSLGFG---AIITYASYVRKDQDIVLSGLTAATLNEKAEVILGGSISIPAAVAFFGVANAVAIAKAGAFNLGFITLPAIFSQTAG-------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3GWU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 117 -24627 -210.49 -304.04 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain A : 0.52 3D Compatibility (PKB) : -210.49 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.52 QMean score : -0.018

(partial model without unconserved sides chains): PDB file : Tito_3GWU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3GWU-query.scw PDB file : Tito_Scwrl_3GWU.pdb: