Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEFTNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDINMGCPVPNVAERGKGSGLILRPEVAAQLIEA-AKAGGLPVSVKTRIG------YTEMSEMEEWISH------LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGEELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSITGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ 3B0P Chain:A ((4-240)) -----------PRLSVAPMVDRTDRHFRFLVRQVSLGVRLYTEMTVDQAVLRGNRERL----LAFRPEEHPIALQLAGSDPKSLAEAARIGEAFGYDEINLNLGCPSEKAQEGGYGACLLLDLARVREILKAMGEAVRVPVTVKMRLGLEGKETYRGLAQSVEAMAEAGVKVFVVHARSAL----------IPPLRHDW-----VHRLKGDF-PQLTFVTNGGI--RSLEEALFHLKRVDGVMLGRAVYEDPFVLEEADR--------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3B0P.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1173 -54575 -46.53 -255.02 target 2D structure prediction score : 0.75 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -46.53 2D Compatibility (Sec. Struct. Predict.) : 0.75 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.489

(partial model without unconserved sides chains): PDB file : Tito_3B0P.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3B0P-query.scw PDB file : Tito_Scwrl_3B0P.pdb: