Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTSGFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHE--EVPRDEKRGQFARASWGIDYHDILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLITEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSYQTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPMLSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECL-TDVRPVIRGTAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK 5T8Y Chain:A ((34-372)) --LKEELIEYAKSIGVDKIGFTTADTFDSLKDRLILQESLGYLSGFEEPDIEKRVTPKLLLPKAKSIVAIALAYPSRMKDAPRSTRTERRGIFCRASWGKDYHDVLREKLDLLEDFLKS------KHEDIRTKSMVDTGELSDRAVAERAGIGFSAKNCMITTPEYGSYVYLAEMITNIPFEPDVPIEDMCGSCTKCLDACPTGALVNPGQLNAQRCISFLTQTKGFLPDEFRTKIGNRLYGCDTCQTVCPLNKGKDFHLHPEMEPDPEIAKPLLKPLLAISNREFKEKFGHVSGSWRGKKPIQRNAILALAHFKDASALPELTELMHKDPRPVIRGTAAWAIGKIG---------------------------------------

General information: TITO was launched using: RESULT: Template: 5T8Y.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1754 19213 10.95 57.18 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.86 3D Compatibility (PKB) : 10.95 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.86 QMean score : 0.515

(partial model without unconserved sides chains): PDB file : Tito_5T8Y.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5T8Y-query.scw PDB file : Tito_Scwrl_5T8Y.pdb: