Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAVMKIEYYSQVLDMEWGVNVLYPDANRVEEPECEDIPVLYLLHGMSGNHNSWLKRTN----VERLLRG---TNLIVVMPNTSNGWYTDT-----QYGFDYYTALAEELPQVLKRFFPNMTSKREKTFIAGLSMGGYGCFKLALT-TNRFSHAASFSGALSFQNFSPESQNLGSPAYWRGVFGEI---RD--WTTSPYSLESLAKKSDKKTKLWAWCGEQDFLYEANNLAVKNLKKLGFDVTYSHSAGTHEWYYWEKQLEVFLTTLPIDFKLEERLT 5VOL Chain:C ((11-272)) -KVVTDTLFSKVLNSKRAYTVFLPKSFEQNKEKK--YPVLYLLHGMWETNPVWAERGHVKDVMDRLVASGEACEMIIVTPNAGGNIHLEWNGYFDMPGWKYETFFYTEFLPYIEKKYR-VIGDRQHRAIAGLSMGGGGATNYGQRHSDMFCAVYAMSALMSIPEQGAVPAD--DPNSKIAILTRSVIENSCVKYVMEADEDRKA--DLRSVAWFVDCGDDDFLLDRNIEFYQAMRNAGVPCQFRVRDGGHDWEYWHSALYQCLPFVTRIF-------

General information: TITO was launched using: RESULT: Template: 5VOL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1330 -26686 -20.06 -109.37 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain C : 0.73 3D Compatibility (PKB) : -20.06 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.457

(partial model without unconserved sides chains): PDB file : Tito_5VOL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5VOL-query.scw PDB file : Tito_Scwrl_5VOL.pdb: