Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MISKRLELVASFVSQGAILLDVGSDHAYLPIELVERGQIKSAIAGEVVEGPYQSAVKNVEAHGLKEKIQVRLANGLAA---FEETDQVSVITIAGMGGRLIA----RILEEGLGKLANVERLILQP-----------NNREDDLRIWLQDHGFQIVAERILEEAGKFYEILVVEAGQMKLSASDVRFGPFLSKEVSPVFVQKWQKEAEKLEFALGQIPEKNLEERQVLVDKIQAIKEVLHVSK 4UW0 Chain:A ((46-191)) -------NLSRALGRPLNVLDLGCAQGFFSLSLASKG--ATIVGIDFQQENINVCRALAEENPD-FAAEFRVGRIEEVIAALEEGE-FDLAIGLSVFHHIVHLHGIDEVKRLLSRLADVTQAVILELAVKEEPLYWGVSQPDDPRELIEQCAFYRLI--------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4UW0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 539 -43244 -80.23 -337.84 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -80.23 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.309

(partial model without unconserved sides chains): PDB file : Tito_4UW0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4UW0-query.scw PDB file : Tito_Scwrl_4UW0.pdb: