Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDLPNPVLAKVTERVIARSQKTRSAYLQRIEHAQGKFPARGALSCANLAHGFAGMEDNEKLIIKVGR-EPNIGIVSSYNEMLSAHAPYKTFPDIIKTAARENGGVAQFAGGVPAMCDGITQGNASMELSLFSRETIAMGTAIALSHNMFDAALCLGVCDKIVPGLLIGALQFGYLPTIFVPAGPMSSGLSNDDK-------AKIRQQFATGQVGRDALLEAESAAYHGQGTCTFYGTANSNQMLMEVMGLHLPSAAFVHPHTPLRDALTAEAAKRVLDLTAEQGNYTPIGHVIDEKAIINGIVALLATGGSTNHTLHLIAIAHAAGILIDWDDFDELSAVVPLLAKIYPNGKADVNHFQAAGGVAFLIRNLLEAGLLHNDVKTVAGKGL-QHYTKEPKLIDGKLTWVDGVVQSLDDKVLRSIDAPFQPDGGLRLMQGRLG--RGVIKISAVAPEHRKVKAPAIVFDSQEAVQAAFDRG--ELHRDFIAVVRFQGARA-NGMPELHR---LTPVLGVLQDQGFHVALVTDGRMSGASGKVPAVIHLSPEALLNGPIGKVQTGDMLIIDAEAGVLDIELDEQTWQSRPVAQPEHQAENEVGFGRELFGVFRAAAAPAEHGASVFGALVGEEPQGQI 5J85 Chain:A ((13-579)) ------------K---------AEWPRKLRSQEWYGGTSR---DVIYHRGWLKNQGYP-HD---LFDGRPVIGILNTWSDMTPCNGHLRELAEKVKAGVWEAGGF-PLEVPVFSASENTFRP-----TAMMYRNLAALAVEEAIRGQPMDGCVLLVGCD-TTPSLLMGAASC-DLPSIVVTGGPMLNGYFRGERVGSGTHLWKFSEMVKAGEMTQAEFLEAEASMSRSSGTCNTMGTASTMASMAEALGMALSGNAAIPGVDSRRKVMAQLTGRRIVQMVK---DDLKPSEIMTKQAFENAIRTNAAIGGSTNAVIHLLAIAGRVGIDLSLDDWDRCGRDVPTIVNLMPSGKYLMEEFFYAGGLPVVLKRLGEAGLLHKDALTVSGETVWDEVKDVV---------------NWNEDVILPAEKALTSSGGIVVLRGNLAPKGAVLKPSAASPHLLVHKGRAVVFEDIDDYKAKINDDNLDIDENCIMVMKNCGPKGYPGMAEVGNMGLPPKVLK--KGILDMV-RISDARMAGTA-YGTVVLHTSPEAAVGGPLAVVKNGDMIELDVPNRRLHLDISDEELARRLA-EWQPNH---DLPTSGYAFLHQQHVEGADTGADLDFLKGCR------

General information: TITO was launched using: RESULT: Template: 5J85.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 3469 -19607 -5.65 -35.71 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -5.65 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.456

(partial model without unconserved sides chains): PDB file : Tito_5J85.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5J85-query.scw PDB file : Tito_Scwrl_5J85.pdb: