Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------------------------------------------------------------MSQSSAALNSPNPMKK---KVITALIAVAIG-----HI-------GVLWAVSHIKPAELKPIDKKPLQVRFVKIQEQPKPLPPKPKEPPKKPEPKKEVKEVKVVEKPVTPPKKVEKIQQVKKAETPKETVKTEP-KVETKVVTTTTTVTEKVVEKPKPVVQEAPKAQPAPDPSPKRVSIGGSGVQWSRS---PRLTVSPKDLQGEARSVVVL-IEADEKGKIIN-----VRVTRSSGISSLDDKVVR-A-------V-RGAKFKPYMENGVAYPIRAEQPFDLNP------------------------------ 5HYZ Chain:A ((1-375)) SAPILQSLLSCSRAAATDPGLAAAELASVRAAATDAGDPSERLAFYFADALSRRLACGASDELTLCYKTLNDACPYSKFAHLTANQAILEATGAATKIHIVDFGIVQGIQWA-ALLQALATRP-EGKPTRIRITGVPSPLLGPQPAASLAATNTRLRDFAKLLGVDFEFVPLLRPVHELNKSDFLVEPDEAVAVNFMLQLYHLLGDSDELVRRVLRLAKSL-------------SPAVVTLGEYEVSLNRAGFVDRFANALSYYRSLFESLDVAMTRDSPERVRVERWMFGERIQRAVGPEEGADRTERMAGSSEWQTLMEWCGFEPVPLSNYARSQADLLLWNYDSKYKYSLVELPPAFLSLAWEKRPLLTVSAWR

General information: TITO was launched using: RESULT: Template: 5HYZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 941 72629 77.18 307.75 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : 77.18 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.121

(partial model without unconserved sides chains): PDB file : Tito_5HYZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5HYZ-query.scw PDB file : Tito_Scwrl_5HYZ.pdb: