Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNSTQQWQSVTWFEVDEHQAGQRIDNFLFSRLKGVPKSRIYRLIREGQVRVNKKRIK-AETKLAIGDQIRVAPIRYEQKDETAAPVSDSVAQGLLSRVVYEDEGLLVVNKPSGIAVHGGSGVAYGLIEALRAATGKKYLELIHRIDRDTSGLVMISKKRSTLKLLQDMLREHKIRKTYAAIVKGQVSLDKQLIDAPLFRYELANGERRVRVSKEGKPSKTEWVVAERFKNATLVHASPLSGRTHQIRVHGLSIGHPLVGDDKYGHNTAYTGPEARRLCLHAMRLDIPGYPTIEAPLPEDMTQLLEALRVAK 1KSK Chain:A ((2-207)) -------------------SHMRLDKFIAQQL-GVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPEHDVAYDGNP---------------------LAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVA-WKLHAAGRLDIDTTGLVLMTDDGQWSHRITSPR--HHCEKTYLVTLESPVAD--DTAEQFAKGVQL-HN-----EKDLTKPAVLEVI------TPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRER-----------------------------------------------

General information: TITO was launched using: RESULT: Template: 1KSK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 837 -5354 -6.40 -26.12 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -6.40 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.293

(partial model without unconserved sides chains): PDB file : Tito_1KSK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1KSK-query.scw PDB file : Tito_Scwrl_1KSK.pdb: