Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFEFVMSEQPISPTVQLNTRGLRCPEPVMMLHQAIRKAK------SG-DVVEVLATDP--------STSWDIPKFCMHLGHELLLKEEVLDEQNHKEYRYLVQKG 2D9I Chain:A ((6-91)) -----------SGQNVLDLHGLHVDEALEHLMRVLEKKTEEFKQNGGKPYLSVITGRGNHSQGGVARIKPAVIKYLISHSFRFSE--I-----KPGCLKVMLKS-

General information: TITO was launched using: RESULT: Template: 2D9I.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 198 -8948 -45.19 -126.03 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -45.19 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.534

(partial model without unconserved sides chains): PDB file : Tito_2D9I.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2D9I-query.scw PDB file : Tito_Scwrl_2D9I.pdb: