Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----MAKSFAINMAP---TNHGGMIPATQSRTSQ-----------EGNLFVRAGDGHMCPKCKCWSVVI-----KSHDHVIMDG---------KPVAYVGDKLSCGATIMPQQFHVVGDSGSPYSSAAPVQ-------NSLVEDKSNEIHKVQFKLVDVDTDQPLSAMLYEIHSKESGKLLVQGYTDKNGMT---AIYESEHTPESVQLITIDLSKPLDPL- 4EGD Chain:A ((27-253)) GRNAQIKDTFNQTLKLYPTKNLDDFYDKEGFRDQEFKKGDKGTWIVNSEMVIEPKGKDM--ETRGMVLYINRNTRTTKGYYFISEMTPKDDEKRYPVKMEHNKIIPTKPLPNDKLKKEIENFKFFVQYGNFKDINDYKDGDISYNPNVPSYSAKYQLNNDDYNVQQLRKRYDIPTKQAPKLLLKGDGDLKGSSVGSRSLEFTFVENKEENIYFTDSVQYTPSE

General information: TITO was launched using: RESULT: Template: 4EGD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 645 41446 64.26 234.16 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : 64.26 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.247

(partial model without unconserved sides chains): PDB file : Tito_4EGD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EGD-query.scw PDB file : Tito_Scwrl_4EGD.pdb: