Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQLSKLLDHWFPHPFVSFLIIIFWLMLAHSLDASDLLMAVILGLVIPRLVRPFITRTPHIHWKPAIKLCFVVLWDIIISNFRVAKLVLGPPKKLHPKWYRVPLETEHEQVNSLLAMIITTTPGTVSAGIDQERGDILVHSLSTENTESDVQDIKQRYE-APLIEIFDVKSKSEDNA 1FEH Chain:A ((1-126)) ----------------MKTIIINGVQFNTDEDTTILKFARDNNIDISALCF-------LNNCNNDINKCEICTVEVEGTGLVTACDTLIEDG-----------------------MIINTN----SDAVNEKIKSRISQLLDIHEFKCGPCNRRENCEFLKLVIKYKARASKPFLP

General information: TITO was launched using: RESULT: Template: 1FEH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 535 -63369 -118.45 -506.95 target 2D structure prediction score : 0.31 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : -118.45 2D Compatibility (Sec. Struct. Predict.) : 0.31 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.228

(partial model without unconserved sides chains): PDB file : Tito_1FEH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1FEH-query.scw PDB file : Tito_Scwrl_1FEH.pdb: