Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --MFTKKDLLQQRMLIDQLRTQNSLS----PQNAA-----KLGQSIASSWERSASAAIPKERFAAPLLEKKSASQ--NALDLALSQCADDLRHI--------------AEQSSMVIAVGDIGST----------IIWTASSAQMQSAAERVHF-----------VQGGQWREEFVGTNALALSL-------------------KTQQSSCVFSNEHYMESIHDWVCYAAPIID---PYSKQTLGVVDLSTT------WKNHNTLGILAAERC-----ASIIQSAL------LEQQRQQLHIRAFST-----PQVKFNGKSLLLT-------------PRQ-------IEIL---TILALCPHGLTLENLYQALYGERKVSMG-------TLKAEMSQLRDILGG--LLGSRPYRLLVHV-----------EADFLQAEQALDAGYVASALQLYAGVFLAKT---ESP---------FLCAWRD--CLESRLSDAIFKAQ--ETDLLLKHLAHFPEAIDAVERLMELLPSEHPAHQLLVKYIDSPKLS------------------------------------------ 1ZRO Chain:A ((8-601)) NEVLSNCREKRKGMKWDCKKKNDRSNYVCIPDRRIQLCIVNLAIIKTYTKETMKDHFIEASKKESQLLLKKNDNKYNSKFCNDLKNSFLDYGHLAMGNDMDFGGYSTKAENKIQEVFKGAHGEISEHKIKNFRKKWWNEFREKLWEAMLSEHKNNICKNIPQEELQITQWIKEWHGEFLLERDNRAKLPKSKCKNNALYEACEKECIDPCMKYRDWIIRSKFEWHTLSKEYETQKVPKENAENYLIKISENKNDAKVSLLLNNCDAEYSKYCDCKHTTTLVKSVLNGNDNTIKEKREHIDLDDFSKFGCDKNSVDTNTKVWECKKPYKLSTKDVCVPPRRQELCLGNIDRIYDKNLLMIKEHILAIA-IYESRILKRKYKNKDDKEVCKIINKTFADIRDIIGGTDYWNDLSNRKLVGKINTNSNYVHRNKQNDKLFRDEWWKVIKKDVWNVISWVFKDKTVCKEDDIENIPQFFRWFSEWGDDYCQDKTKMIETLKVECCEDDNCKRKCNSYKEWISKKKEEYNKQAKQYQEYQKGNNYKMYSEFKSIKPEVYLKKYSEKCSNLNFEDEFKEELHSDYKNKCTMCPEV

General information: TITO was launched using: RESULT: Template: 1ZRO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1544 97975 63.46 248.04 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 63.46 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.231

(partial model without unconserved sides chains): PDB file : Tito_1ZRO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ZRO-query.scw PDB file : Tito_Scwrl_1ZRO.pdb: