Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHCPFCNAADSKVIDSRLAAEGCQIRRRRECVSCGERFTTFESYEVVMPRVIKSNGKNEPFDEAKLRRSLMHALQKRPVT--------QEQIETVLSDIQLQIRRL--GERDVKSRTIGEIVMQSLFALDHVAYVRFASVYQDFQDVEAFRRQIEQMQQREH 5IM3 Chain:A ((32-143)) ------------------------------------------------LRVIKRNGTVVPYTDDKITVAITKAFLAVEGGTAAASSRIHDTVRRLTEQVTATFKRRMPSGGTIHIEEIQDQVELALMRA---GEQKVARDYVIYREARAAERKNAG------

General information: TITO was launched using: RESULT: Template: 5IM3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 304 -3971 -13.06 -41.80 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -13.06 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.653

(partial model without unconserved sides chains): PDB file : Tito_5IM3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5IM3-query.scw PDB file : Tito_Scwrl_5IM3.pdb: