Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHIKEKTLDDLLNSTYTFILENGDHITA-SKGENFEVRNAVLTLENPRSRLSISE-TRSKLISCIGEFFWYLNGSDSLEFIEYYISDYRK-YIEYQIDENKPALGAYGPRIFGA-------QNQFNKIIEVLKDKPTSRRAVISIYSKDDLLREDSRDIPCTCLLQFFIRNERLHLTATMRSNDAAIGLVHDIFSFTLIQELLLAKLIRYIPNLQLGEYTHIVGSLHIYKKI 1TJS Chain:A ((14-213)) --------------------DEGTQKNDRTGTGTLSIFGHQMRFNL-QDGFPLVTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWAD----ENGDLGPVYGKQWRAWPTPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELD--KMALAPCHAFFQFYVADGKLSCQLYQRSCDVFLGLPFNIASYALLVHMMAQ-----QCDLEVGDFVWTGGDTHLYSNH

General information: TITO was launched using: RESULT: Template: 1TJS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 754 -21485 -28.49 -113.08 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -28.49 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.359

(partial model without unconserved sides chains): PDB file : Tito_1TJS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1TJS-query.scw PDB file : Tito_Scwrl_1TJS.pdb: